Sequential passage of influenza virus in embryonated eggs or tissue culture: Emergence of mutants
Identifieur interne : 002799 ( Main/Exploration ); précédent : 002798; suivant : 002800Sequential passage of influenza virus in embryonated eggs or tissue culture: Emergence of mutants
Auteurs : Colin Brand [États-Unis] ; Peter Palese [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1980.
English descriptors
- Teeft :
- Clone, Embryonated, Embryonated eggs, Genome, Independent lines, Influenza, Influenza virus, Influenza virus mutants, Influenza viruses, Mdbk, Mdbk cells, Mdck, Mdck cells, Mutant, Mutation, Mutational changes, Oligonucleotide, Oligonucleotide changes, Oligonucleotide mapping, Oligonucleotide maps, Oligonucleotide patterns, Oligonucleotides, Open circles, Original preparation, Palese, Parent clone, Parent preparation, Passage level, Passaged, Passaging, Rna, Seed virus, Spontaneous mutation, Stomatitis, Terminal passage, Tissue culture, Vesicular, Vesicular stomatitis virus, Virus, Virus clones, Virus passaged, Virus preparation.
Abstract
Abstract: The emergence of influenza virus mutants was examined by passage of A/WSN/33 virus in embryonated eggs, in MDBK cells, and in MDCK cells. From a plaque-purified A/WSN/33 virus preparation, four independent clones were derived by 12 plaque-to-plaque passages in MDCK cells. Four clones were isolated following 8–12 plaque-to-plaque passages of A/WSN/33 virus in MDBK cells, and one clone was derived by 12 passages in embryonated eggs at limiting dilution. All nine clones independently derived by these passages showed differences in their oligonucleotide maps when compared to the map of the original preparation of plaque-purified A/WSN/33 virus. In contrast, 12 passages in embryonated eggs at low dilutions (10'-10' PFU per inoculum) resulted in an A/WSN/33 virus population which was indistinguishable by oligonucleotide mapping from the original preparation. The frequent emergence of influenza A virus variants upon plaque-to-plaque passage contrasts with the finding that three out of four vesicular stomatitis virus clones, which were derived from independent plaque-to-plaque passage in MDCK cells, were identical to the original preparation when compared by oligonucleotide mapping.
Url:
DOI: 10.1016/0042-6822(80)90309-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000B00
- to stream Istex, to step Curation: 000B00
- to stream Istex, to step Checkpoint: 001413
- to stream Main, to step Merge: 002946
- to stream Main, to step Curation: 002799
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Sequential passage of influenza virus in embryonated eggs or tissue culture: Emergence of mutants</title><author><name sortKey="Brand, Colin" sort="Brand, Colin" uniqKey="Brand C" first="Colin" last="Brand">Colin Brand</name></author><author><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6787A44CC3E4A0426C96B685CB35D29D42DB42A9</idno><date when="1980" year="1980">1980</date><idno type="doi">10.1016/0042-6822(80)90309-8</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-W3S5XQ8X-8/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000B00</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B00</idno><idno type="wicri:Area/Istex/Curation">000B00</idno><idno type="wicri:Area/Istex/Checkpoint">001413</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001413</idno><idno type="wicri:doubleKey">0042-6822:1980:Brand C:sequential:passage:of</idno><idno type="wicri:Area/Main/Merge">002946</idno><idno type="wicri:Area/Main/Curation">002799</idno><idno type="wicri:Area/Main/Exploration">002799</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Sequential passage of influenza virus in embryonated eggs or tissue culture: Emergence of mutants</title><author><name sortKey="Brand, Colin" sort="Brand, Colin" uniqKey="Brand C" first="Colin" last="Brand">Colin Brand</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mount Sinai School of Medicine of CUNY, Fifth Avenue and 100th Street, New York, New York 10029</wicri:regionArea><wicri:noRegion>New York 10029</wicri:noRegion></affiliation></author><author><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mount Sinai School of Medicine of CUNY, Fifth Avenue and 100th Street, New York, New York 10029</wicri:regionArea><wicri:noRegion>New York 10029</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">107</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="424">424</biblScope><biblScope unit="page" to="433">433</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Clone</term><term>Embryonated</term><term>Embryonated eggs</term><term>Genome</term><term>Independent lines</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus mutants</term><term>Influenza viruses</term><term>Mdbk</term><term>Mdbk cells</term><term>Mdck</term><term>Mdck cells</term><term>Mutant</term><term>Mutation</term><term>Mutational changes</term><term>Oligonucleotide</term><term>Oligonucleotide changes</term><term>Oligonucleotide mapping</term><term>Oligonucleotide maps</term><term>Oligonucleotide patterns</term><term>Oligonucleotides</term><term>Open circles</term><term>Original preparation</term><term>Palese</term><term>Parent clone</term><term>Parent preparation</term><term>Passage level</term><term>Passaged</term><term>Passaging</term><term>Rna</term><term>Seed virus</term><term>Spontaneous mutation</term><term>Stomatitis</term><term>Terminal passage</term><term>Tissue culture</term><term>Vesicular</term><term>Vesicular stomatitis virus</term><term>Virus</term><term>Virus clones</term><term>Virus passaged</term><term>Virus preparation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The emergence of influenza virus mutants was examined by passage of A/WSN/33 virus in embryonated eggs, in MDBK cells, and in MDCK cells. From a plaque-purified A/WSN/33 virus preparation, four independent clones were derived by 12 plaque-to-plaque passages in MDCK cells. Four clones were isolated following 8–12 plaque-to-plaque passages of A/WSN/33 virus in MDBK cells, and one clone was derived by 12 passages in embryonated eggs at limiting dilution. All nine clones independently derived by these passages showed differences in their oligonucleotide maps when compared to the map of the original preparation of plaque-purified A/WSN/33 virus. In contrast, 12 passages in embryonated eggs at low dilutions (10'-10' PFU per inoculum) resulted in an A/WSN/33 virus population which was indistinguishable by oligonucleotide mapping from the original preparation. The frequent emergence of influenza A virus variants upon plaque-to-plaque passage contrasts with the finding that three out of four vesicular stomatitis virus clones, which were derived from independent plaque-to-plaque passage in MDCK cells, were identical to the original preparation when compared by oligonucleotide mapping.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Brand, Colin" sort="Brand, Colin" uniqKey="Brand C" first="Colin" last="Brand">Colin Brand</name></noRegion><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002799 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002799 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:6787A44CC3E4A0426C96B685CB35D29D42DB42A9
|texte= Sequential passage of influenza virus in embryonated eggs or tissue culture: Emergence of mutants
}}
| This area was generated with Dilib version V0.6.33. |  |